XenoLight™ RediJect D-Luciferin Ultra

RediJect D-Luciferin Ultra (K+ salt) is a novel bioluminescence substrate in a ready to use injectable format. Rediject D-Luciferin Ultra is pre-formulated with a fluorescent marker to validate your substrate injection. With Caliper's Rediject D-Luciferin Ultra you can ensure reproducible results by minimizing variability due to formulation and substrate injection, minimizing freeze/thaw and controlling batch to batch variation.

Rediject D-Luciferin Ultra is pre-formulated with a rapidly clearing fluorescent dye to validate the integrity of your substrate injection, so with a quick fluorescent image on your IVIS system you know immediately if an injection failed and you can normalize your bioluminescence results. With Rediject D-Luciferin Ultra, you can have extra confidence in your data quality.

• Pre-formulated, batch controlled D-Luciferin for in vivo use
• Instantly validate your injection quality and minimize data variability
• Save substantial time and effort by minimizing pre-imaging preparation steps
• Dispensed to image 5 animals per vial* (10 vials/kit) 
• In vivo imaging quality, validated on IVIS imaging systems
   

Color and Form:  Yellow colored solution (D-Luciferin Potassium salt in PBS)
Concentration:  30 mg/mL
Volume per vial:  10 sterile vials each containing 850 μL of 30 mg/ml D-Luciferin
Storage and Handling:  Store at ≤ -70 °C. Just before your experiment, thaw required number of vials in a 37 °C water bath, vortex and it is ready to use. Repeated freeze thaw is not recommended. Rediject D-Luciferin supports intravenous, subcutaneous or intraperitoneal injection.

For in vivo imaging studies, we recommend intraperitoneal injection at 150 mg/kg (150 μL /mouse*). Load a 1 mL syringe directly from the vial and inject using a 25 gauge needle. For Fluorescence imaging, select exposure time between 1 and 5 seconds and image using 745 nm excitation and 800 nm emission filter set (ICG filter set for IVIS Lumina). Select an ROI on the scruff of the neck on the dorsal side to evaluate the accuracy of your intraperitoneal injection. For ventral imaging, ROI should be drawn around the thoracic region. * Calculations based on a 30g mouse